Reporter

Part:BBa_K3500001:Design

Designed by: Kennex Lam   Group: iGEM20_GA_State_SW_Jiaotong   (2020-10-26)


GUS Gene Codon Optimized for S. microadriaticum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 370
    Illegal NgoMIV site found at 502
    Illegal NgoMIV site found at 1099
    Illegal NgoMIV site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 16
    Illegal BsaI.rc site found at 1051
    Illegal BsaI.rc site found at 1147
    Illegal BsaI.rc site found at 1435
    Illegal SapI.rc site found at 1731


Design Notes

Codon optimized GUS gene for Symbiodinium microadriaticum.


Source

ten Lohuis, M., & Miller, D. . (1998). Genetic transformation of dinoflagellates (Amphidinium and Symbiodinium): expression of GUS in microalgae using heterologous promoter constructs. The Plant Journal, 13(3), 427-435. doi:10.1046/j.1365-313X.1998.00040.x

Chen, P. Y., Wang, C. K., Soong, S. C., & To, K. Y. (2003). Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Molecular breeding, 11(4), 287-293. Retieved from https://link.springer.com/article/10.1023/A:1023475710642


References